| Player | Active | Position | Remark |
|---|---|---|---|
| Andre Onana | Start | Keeper | |
| Rasmus Nissen | Start | Defender | |
| Matthijs de Ligt | Start | Defender | |
| Perr Schuurs | Start | Defender | |
| Noussair Mazraoui | Start | Defender | |
| Mitchell Bakker | Start | Midfielder | |
| Jurgen Ekkelenkamp | Start | Midfielder | |
| Donny van de Beek | Start | Midfielder | |
| David Neres | Start | Forward | |
| Kasper Dolberg | Start | Forward | |
| Justin Kluivert | Start | Forward | |
| Norbert Alblas | Bench | ||
| Kostas Lamprou | Bench | ||
| Dennis Johnsen | Bench | ||
| Mateo Cassierra | Bench | ||
| Luis Orejuela | Bench | ||
| Dani de Wit | Bench | ||
| Hassane Bande | Bench | ||
| Teun Bijleveld | Bench | ||
| Azor Matusiwa | Bench |
| Player | Active | Remark |
|---|---|---|
| Vaclav Cerny | Injured | |
| Carel Eiting | Injured | |
| Klaas-Jan Huntelaar | Injured | |
| Frenkie de Jong | Injured | |
| Siem de Jong | Injured | |
| Benjamin van Leer | Injured | |
| Lasse Schöne | International duties | |
| Daley Sinkgraven | Injured | |
| Nicolás Tagliafico | International duties | |
| Joël Veltman | Injured | |
| Nick Viergever | Injured | |
| Max Wöber | Injured | |
| Amin Younes | Removed disciplinary | |
| Hakim Ziyech | International duties |
| Al-Ahly | Minute | Ajax |
|---|---|---|
| Second half | ||
| 46 | Substistuted Justin Kluivert out, in Hassane Bande | |
| 46 | Substistuted Donny van de Beek out, in Azor Matusiwa | |
| 46 | Substistuted Andre Onana out, in Norbert Alblas | |
| 50 | Substistuted Noussair Mazraoui out, in Teun Bijleveld | |
| 50 | Substistuted Jurgen Ekkelenkamp out, in Dani de Wit | |
| 63 | Substistuted Kasper Dolberg out, in Mateo Cassierra | |
| 63 | Substistuted Matthijs de Ligt out, in Luis Orejuela | |
| 75 | Substistuted David Neres out, in Dennis Johnsen | |
| Goal Ahmed Ramdan, after attack | 83 |